The CD11b + /Ly6C med /Ly6G + myeloid cells represent the granulocytic subset of a heterogeneous class of myeloid cells termed myeloid-derived suppressor cells (MDSCs; ref. 16). The monocytic fraction (CD11b + /Ly6C high /Ly6G −) suppresses CD8 + T cell and NK cell function, but the role of the granulocytic fraction is still unclear (16).

The RB6-8C5 monoclonal antibody reacts strongly with mouse Ly6G and weakly with mouse Ly6C previously referred to as GR-1. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion.

The Ly6C + Ly6G-(top, open histograms) and Ly6C + Ly6G + (bottom, open histograms) CD11b + monocyte subpopulations were analyzed for the expression of various cytokines/effector molecules using antibodies (A-D) or a reactive dye triggered by exposure to ROS (E) and compared to CD11b-cells (filled histograms). For TNF-α staining, cells were (A) Quantification of flow cytometry reveals that, whereas CD11b + Ly6G + neutrophils are unaffected at 1 or 3 d after CFA injection (P ≥ 0.329), there is a significant reduction in (CD11b + Ly6G −) Ly6C hi and Ly6C low cells at 1 and 3 d and in Ly6C med cells at 3 d only (1 d, n = 3–4 per group; 3 d, n = 5–7 per group). The Ly6C+Ly6G- (top, open histograms) and Ly6C+Ly6G+ (bottom, open histograms) CD11b+ monocyte subpopulations were analyzed for the expression of various cytokines/effector molecules using antibodies (A-D) or a reactive dye triggered by exposure to ROS (E) and compared to CD11b- cells (filled histograms). B) Percentage of CD11b + Gr1 + cells of total CD45 + cells isolated from murine livers following Hx or sham laparotomy, as determined by flow cytometry on indicated PODs. C) Representative plots from d 2 of data presented in B. D) Fold change (shown as log2) of the percentage of Ly6G + or Ly6C + of CD11b + cells in murine livers of Hx vs. sham.

Research output: Contribution to journal › Article Samples were stained concurrently with two different cocktails. The first cocktail contained CD11b, Ly6C, Ly6G, CD11c, and F4/80 (Fig. 3a) while the second cocktail contained CD11b, Ly6C, Ly6G, CD11c, and NK1.1 (Fig. 3b). After gating out debris, doublets, and nonviable cells, four sub‐populations of CD11b + cells were sorted.

2020-06-19 · In the myeloid gate (CD11b + CD172a +), neutrophils are Ly6G +, eosinophils are Siglec F +, monocytes are Siglec F − Ly6G − CD115 + and form a continuum from Ly6C hi to Ly6C lo. In the lymphoid gate (CD172a − CD11b lo-neg ), B cells are CD19 + MHCII + , T cells are CD19 − CD3e + , NK cells are CD19 − CD3e − NK1.1 + . The Ly6C+Ly6G- (top, open histograms) and Ly6C+Ly6G+ (bottom, open histograms) CD11b+ monocyte subpopulations were analyzed for the expression of various cytokines/effector molecules using antibodies (A-D) or a reactive dye triggered by exposure to ROS (E) and compared to CD11b- cells (filled histograms).

Myeloid-derived cells have been implicated as playing essential roles in cancer therapy, particularly in cancer immunotherapy. Most studies have focused on either CD11b+Ly6G+Ly6C+ granulocytic or polymorphonuclear myeloid-derived suppressor cells (G-MDSCs or PMN-MDSCs) or CD11b+Ly6G−Ly6C+ monocytic MDSCs (M-MDSCs), for which clear roles have been established.

Most studies have focused on either CD11b+Ly6G+Ly6C+ granulocytic or polymorphonuclear myeloid-derived suppressor cells (G-MDSCs or PMN-MDSCs) or CD11b+Ly6GLy6C+ monocytic MDSCs (M-MDSCs), for which clear roles have been established. On the other hand, CD11b+Ly6GLy6C myeloid-derived cells (MDCs) have been less well studied. CD11b(+)Ly6C(++)Ly6G(-) cells show distinct function in mice with chronic inflammation or tumor burden.

developing bone marrow macrophages; including CD11b CD31 CD44 Ly6C Ly6G F4/80 Mac2 c-Fos as well as c-Fms. In addition RNA was preserved during

Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These 2015-12-01 2006-01-15 Cd11b+Gr1+ cells and identify subpopulations accumulating in the premetastatic lungs (Fig. 1D). Because the anti-Gr1 antibody Ly6G-Ly6C- cells, suggesting that the primary tumor secretes fac-tors that speciﬁcally up-regulate Bv8 expression in Ly6G+Ly6C+ granulocytes 2020-06-02 cells from pristane-treated mice were st ained with anti-CD11b and anti-Ly6C and sorted using a FACSAria (BD Biosciences) into CD11b +Ly6Chi and CD11b Ly6Cmid populations. In addition, splenocytes were stained with anti-CD11b and anti-Ly6C and sorted into CD11b+Ly6Chi cells.

2016-11-11 · CD11b+Ly6G+ cells that had been immunosuppressive myeloid cells. 25 Administration of ligands for TLR3 or TLR9 induces a functional conversion of CD11b + Gr1 + MDSCs or CD11b + Ly6G − Ly6C (B) CD11b + Ly6G − myeloid cells can be separated into three populations based on Ly6C expression, with Ly6C low myeloid cells constituting the bulk of these cells in the naïve animal.
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For example, the staining intensity of GR1 on neutrophils (CD11b + GR1 hi) drops upon anti-Ly6G administration Colorectal immunocytes were stained with anti-CD11b+ Ly6C+ (101206, 128008, Biolegend) to determine monocytic myeloid-derived suppressor cells (M-MDSCs), anti-CD11b+ Ly6G+ (101206, 1127627, Biolegend) to granulocytic myeloid-derived suppressor cells (G-MDSC).

We observed a marked enrichment (ﬁve- to six-fold) in Ly6C+Ly6G+ granulocytes and a modest accumulation of Ly6C+Ly6G- monocytes and F4/80+ macrophages in the premetastatic lungs of 4T1 tumor-bearing mice (Fig. 1D anti-Ly6C antibodies, which divide CD11b+Gr-1+ MDSCs into Ly6G + granulocytes and Ly6C monocytes (33).
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Samples were stained concurrently with two different cocktails. The first cocktail contained CD11b, Ly6C, Ly6G, CD11c, and F4/80 (Fig. 3a) while the second cocktail contained CD11b, Ly6C, Ly6G, CD11c, and NK1.1 (Fig. 3b). After gating out debris, doublets, and nonviable cells, four sub‐populations of CD11b + cells were sorted.

16). The monocytic fraction (CD11b + /Ly6C high /Ly6G −) suppresses CD8 + T cell and NK cell function, but the role of the granulocytic fraction is still unclear (16).